Laboratory equipment and facilities

Laboratory Equipment and Facilities

IHMRI’s laboratories have been designed to host a broad range of basic science research. Facilities include:

  • six large open-plan laboratory modules
  • primary and established tissue culture areas
  • dedicated bacterial culture area
  • two large common equipment areas
  • two darkrooms
  • two cold rooms; each incorporating storage and work areas
  • four constant temperature rooms
  • three microscopy rooms
  • dedicated, restricted-access medium level radiation work area
  • -80 degree freezer holding rooms
  • two cryogenic storage rooms
  • dedicated, restricted-access quarantine facility
  • reticulated gas and vacuum throughout the building
  • reticulated distilled and Milli-Q water supply to laboratory modules.

Equipment

Our laboratories are also home to some of the latest equipment for scientific and medical research. Major equipment is listed below by category.

Category

Equipment
Automation 1 x Liquid handling workstation (Hamilton STAR 60)
1 x Nanopipettor (XYZ Robotic, Nanolitre Dispenser)
Bacterial Culture 4 x Biosafety cabinets (Class II)
1 x Cell density meter (Biochrom, C08000)
1 x CO2 incubator (NuAir)
1 x Gene pulser (Bio-Rad)
Miscellaneous orbital shakers and incubators
Cellular Analysis
1 x Cell analyzer (Roche, RTCA DP Xcelligence)
Centrifugation
1 x Eppendorf (5810R)
1 x Heraeus (Fresco 21)
1 x Heraeus (Labofuge 400R)
2 x Heraeus (Megafuge 1.0R)
1 x Heraeus (Biofuge Primo)
3 x Heraeus (Multifuge X3R)
1 x Heraeus (Multifuge X1R)
2 x Sorvall (RC 6+)
1 x Sorvall (MTX150)
Controlled Temperature Rooms 4 x Constant temperature rooms
2 x Cold rooms
Chromatography
1 x HPLC (Waters)
2 x MPLC (Bio-Rad, NGC Quest 10) 
1 x LPLC (Bio-Rad, Profinia)
1 x LPLC (Bio-Rad, BioLogic)
Dark Room 1 x Film processor (AGFA, CP1000)
2 x Silver recovery units
2 x Dark room facilities
Digital Imaging
1 x Molecular imager (Carestream, Gel Logic 2200 PRO) 
1 x Molecular imager (Carestream, Gel Logic 212 PRO)
1 x Molecular imager (Amersham, Imager 600)
1 x Cell imager (Essen Bioscience, IncuCyte ZOOM)
Flow Cytometry
1 x BD LSR-II (equipped with 3 lasers)
1 x BD LSR Fortessa X-20 (equipped with 4 lasers and FACSFlow System)
1 x Cell sorter (Bio-Rad, S3)
General Laboratory Equipment
 
1 x Analytical balance (Mettler Toledo, XS105DU)
7 x Fumehoods (Conditionaire, Safe-tee Series 2000)
2 x Glassware dishwashers (Miele)
2 x Glassware drying ovens
1 x Label printer (Zebra, GK420t)
2 x Ice machines
12 x Vacuum pumps (Welch)
1 x Vacuum pump (Vacuubrand)
Reticulated gas (Argon, carbon dioxide, nitrogen)
Miscellaneous fridges and freezers
Miscellaneous incubators, rockers and shakers
Miscellaneous microscopes
Miscellaneous microwaves
Miscellaneous water baths
Histology 1 x Automated vacuum tissue processor (Leica, ASP 2005)
2 x Cryostats (Leica, CM1950)
1 x Cryostat (ThermoFisher, Cryotome E)
1 x Freeze dryer (Christ, ALPHA 1-2 LD plus)
1 x Linear Stainer (Leica, ST4020)
1 x Rotary microtome (Leica, RM2255)
1 x Paraffin embedding station (Leica, EG1150H)
Mass Spectrometry and related
1 x GC-MS (Agilent, Triple Quadrupole 7000A)
1 x LC-MS (ThermoFisher, LCQ Fleet)
Access to many other leading-edge MS and NMR machines via the School of Chemistry
Microplate Readers
 
1 x FLUOstar (BMG OPTIMA)
2 x POLARstar (BMG OMEGA)
2 x SpectraMax (Molecular Devices 384 Plus) 
Microscopy
1 x Confocal microscope (Leica, TCS SP5 Advanced System - UV-VIS-IR and X1-Port Access with SMD FCS and CO2 incubation chamber)
1 x Fluorescence microscope (Nikon, TE2000-E with cell incubator chamber)
1 x Fluorescence microscope (Leica, DMi8 with cell incubation chamber)
1 x Microscope camera (Moticam 2000)
1 x Microscope camera (Leica, DM750 with ICC50)
Various basic microscopes
Molecular Analysis 1 x Electrophoresis bioanalyser (Agilent, 2100)
1 x Flow cell fluometry (Abacus, Luminex LX100)
1 x Isothermal titration calorimetry (TA instruments, Nano-ITC)
1 x Particle analyser (Malvern, Zetasizer APS)
1 x Particle analyser (Nanosight, LM10)
1 x Spectrophotometer (ThermoFisher, NanoDrop 2000c)
PCR 3 x PCR (Eppendorf, Mastercycler Pro S)
1 x RT-PCR (Roche, LightCycler 480 II)
Radiation
1 x Beta counter (Perkin Elmer, Tri-Carb 2810 TR)
1 x Beta imager 2000 (Biospace)
1 x Contamination monitor (Mini Instruments, 900 series with EP15 probe)
1 x Contamination monitor (ThermoFisher, RadEye B20-ER)
1 x Gamma counter (Perkin Elmer, Wallac 1480)
1 x Fume hood (Conditionaire, Safe-tee Series 2000)
Purpose-built radiation room (medium level)
Sample Preparation
1 x Freeze dryer (Christ, ALPHA 1-2 LD plus)
1 x Homogeniser (Avestin, C5)
2 x Sonicators (Branson, 250) 
Sterilisation
2 x Autoclaves (PC2) (Getinge, H5 5510 EC1)
1 x Autoclave (PC3) (Getinge, GE 6610 ERC-2)
Tissue Culture
8 x Biosafety cabinets (Class II, various)
1 x Cytotoxic cabinet (Safemate, 1.2 Cyto)
1 x Laminar flow cabinet (Purair)
10 x CO2 incubator (HERAcell)
4 x CO2/O2 incubator (HERAcell)
2 x Liquid nitrogen storage dewars
2 x Liquid nitrogen dispensing dewars
1 x Liquid nitrogen vapour storage vessel (Capacity - 33,684 samples)
1 x Electrical stimulator (Global BioTech, ISO-Flex Isolator and Master-9 pulse stimulator
Ultra Low Temperature Freezers
6 x -80C freezers (Forma, 900 series)
1 x -80C freezers (Forma, TSU700 series)
1 x freezer monitoring system (Testo, Saveris)
Water Purification System
4 x Milli-Q water pods (Millipore, Advantage A10)

 

Last reviewed: 27 October, 2015